A statistical strategy to identify recombinant viral ribonucleoprotein of avian, human, and swine influenza A viruses with elevated polymerase activity
Identifieur interne : 000922 ( Main/Exploration ); précédent : 000921; suivant : 000923A statistical strategy to identify recombinant viral ribonucleoprotein of avian, human, and swine influenza A viruses with elevated polymerase activity
Auteurs : Alex W. H. Chin ; Benjamin D. Greenbaum ; Olive T. W. Li ; Richard J. Webby ; Leo L. M. PoonSource :
- Influenza and Other Respiratory Viruses [ 1750-2640 ] ; 2013.
Abstract
Reassortment of influenza
Eighty one chimeric v
It was found that
Statistical approach and fractional factorial design are useful to identify the major single subunit or interaction factors that can modulate viral polymerase activity.
Url:
DOI: 10.1111/irv.12117
PubMed: 23634764
PubMed Central: 3823691
Affiliations:
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viruses with elevated polymerase activity</title>
<author><name sortKey="Chin, Alex W H" sort="Chin, Alex W H" uniqKey="Chin A" first="Alex W. H." last="Chin">Alex W. H. Chin</name>
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<author><name sortKey="Greenbaum, Benjamin D" sort="Greenbaum, Benjamin D" uniqKey="Greenbaum B" first="Benjamin D." last="Greenbaum">Benjamin D. Greenbaum</name>
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<author><name sortKey="Li, Olive T W" sort="Li, Olive T W" uniqKey="Li O" first="Olive T. W." last="Li">Olive T. W. Li</name>
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<author><name sortKey="Webby, Richard J" sort="Webby, Richard J" uniqKey="Webby R" first="Richard J." last="Webby">Richard J. Webby</name>
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</affiliation>
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<author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">A statistical strategy to identify recombinant viral ribonucleoprotein of avian, human, and swine influenza <styled-content style="fixed-case">A</styled-content>
viruses with elevated polymerase activity</title>
<author><name sortKey="Chin, Alex W H" sort="Chin, Alex W H" uniqKey="Chin A" first="Alex W. H." last="Chin">Alex W. H. Chin</name>
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<author><name sortKey="Greenbaum, Benjamin D" sort="Greenbaum, Benjamin D" uniqKey="Greenbaum B" first="Benjamin D." last="Greenbaum">Benjamin D. Greenbaum</name>
<affiliation><nlm:aff id="irv12117-aff-0002"></nlm:aff>
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<author><name sortKey="Li, Olive T W" sort="Li, Olive T W" uniqKey="Li O" first="Olive T. W." last="Li">Olive T. W. Li</name>
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<author><name sortKey="Webby, Richard J" sort="Webby, Richard J" uniqKey="Webby R" first="Richard J." last="Webby">Richard J. Webby</name>
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<author><name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name>
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<series><title level="j">Influenza and Other Respiratory Viruses</title>
<idno type="ISSN">1750-2640</idno>
<idno type="eISSN">1750-2659</idno>
<imprint><date when="2013">2013</date>
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<front><div type="abstract" xml:lang="en"><sec id="irv12117-sec-0001"><title>Objectives</title>
<p>Reassortment of influenza <styled-content style="fixed-case">A</styled-content>
viruses can give rise to viral ribonucleoproteins (v<styled-content style="fixed-case">RNP</styled-content>
s) with elevated polymerase activity and the previous three pandemic influenza viruses contained reassorted v<styled-content style="fixed-case">RNP</styled-content>
s of different origins. These suggest that reassorted v<styled-content style="fixed-case">RNP</styled-content>
may be one of the factors leading to a pandemic virus. In this study, we reconstituted chimeric v<styled-content style="fixed-case">RNP</styled-content>
s with three different viral strains isolated from avian, human and swine hosts. We applied a statistical strategy to identify the effect that the origin of a single v<styled-content style="fixed-case">RNP</styled-content>
protein subunit or the interactions between these subunits on polymerase activity.</p>
</sec>
<sec id="irv12117-sec-0002"><title>Design</title>
<p>Eighty one chimeric v<styled-content style="fixed-case">RNP</styled-content>
s were reconstituted in 293<styled-content style="fixed-case">T</styled-content>
cells at different temperatures. Polymerase activity was determined by luciferase reporter assay and the results were analysed by multiway <sc>anova</sc>
and other statistical methods.</p>
</sec>
<sec id="irv12117-sec-0003"><title>Results</title>
<p>It was found that <styled-content style="fixed-case">PB</styled-content>
2, <styled-content style="fixed-case">PB</styled-content>
1, <styled-content style="fixed-case">NP</styled-content>
,<styled-content style="fixed-case"> PB</styled-content>
2‐<styled-content style="fixed-case">PB</styled-content>
1 interaction, <styled-content style="fixed-case">PB</styled-content>
2‐<styled-content style="fixed-case">PA</styled-content>
interaction and <styled-content style="fixed-case">PB</styled-content>
1‐<styled-content style="fixed-case">NP</styled-content>
interaction had significant effect on polymerase activity at 37°C and several single subunits and interactions were identified to lead to elevation of polymerase activity. Furthermore, we studied 27 out of these 81 different chimieric v<styled-content style="fixed-case">RNP</styled-content>
s in different combinations via fractional factorial design approach. Our results suggested that the approach can identify the major single subunit or interaction factors that affect the polymerase activity without the need to experimentally reproduce all possible v<styled-content style="fixed-case">RNP</styled-content>
combinations.</p>
</sec>
<sec id="irv12117-sec-0004"><title>Conclusions</title>
<p>Statistical approach and fractional factorial design are useful to identify the major single subunit or interaction factors that can modulate viral polymerase activity.</p>
</sec>
</div>
</front>
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<name sortKey="Greenbaum, Benjamin D" sort="Greenbaum, Benjamin D" uniqKey="Greenbaum B" first="Benjamin D." last="Greenbaum">Benjamin D. Greenbaum</name>
<name sortKey="Li, Olive T W" sort="Li, Olive T W" uniqKey="Li O" first="Olive T. W." last="Li">Olive T. W. Li</name>
<name sortKey="Poon, Leo L M" sort="Poon, Leo L M" uniqKey="Poon L" first="Leo L. M." last="Poon">Leo L. M. Poon</name>
<name sortKey="Webby, Richard J" sort="Webby, Richard J" uniqKey="Webby R" first="Richard J." last="Webby">Richard J. Webby</name>
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